نقش فنولیک های دیواره سلولی
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نقش فنولیک های دیواره سلولی

عنوان فارسی مقاله: نقش فنولیک های دیواره سلولی در بازسازی اولیه سلول های ذرت دارای کمبود سلولز
عنوان انگلیسی مقاله: The role of cell wall phenolics during the early remodelling of cellulose-deficient maize cells
مجله/کنفرانس: فیتوشیمی – Phytochemistry
رشته های تحصیلی مرتبط: زیست شناسی، کشاورزی
گرایش های تحصیلی مرتبط: علوم گیاهی، زراعت و اصلاح نباتات
کلمات کلیدی فارسی: ذرت (.Zea mays L)، چمنیان (Poaceae)، ذرت، کروماتوگرافی مایع با کارایی بالا (HPLC)، هیدروکسی سینامات ها، دیواره سلولی، اسید p-coumaric، اسید فرولیک، دیکلوبنیل
کلمات کلیدی انگلیسی: Zea mays L.، Poaceae، Maize، HPLC، Hydroxycinnamates، Cell wall، p-coumaric acid، Ferulic acid، Dichlobenil
نوع نگارش مقاله: مقاله پژوهشی (Research Article)
نمایه: Scopus – Master Journals List – JCR
شناسه دیجیتال (DOI): https://doi.org/10.1016/j.phytochem.2019.112219
دانشگاه: Área de Fisiología Vegetal, Departamento de Ingeniería y Ciencias Agrarias, Universidad de León, E-24071, León, Spain
ناشر: الزویر - Elsevier
نوع ارائه مقاله: ژورنال
نوع مقاله: ISI
سال انتشار مقاله: 2020
ایمپکت فاکتور: 3.027 در سال 2019
شاخص H_index: 157 در سال 2020
شاخص SJR: 0.926 در سال 2018
شناسه ISSN: 0031-9422
شاخص Quartile (چارک): Q1 در سال 2018
فرمت مقاله انگلیسی: PDF
تعداد صفحات مقاله انگلیسی: 8
وضعیت ترجمه: ترجمه نشده است
قیمت مقاله انگلیسی: رایگان
آیا این مقاله بیس است: خیر
آیا این مقاله مدل مفهومی دارد: ندارد
آیا این مقاله پرسشنامه دارد: ندارد
آیا این مقاله متغیر دارد: ندارد
کد محصول: E14149
رفرنس: دارای رفرنس در داخل متن و انتهای مقاله
فهرست انگلیسی مطالب

Abstract


Graphical abstract


Abbreviations


۱٫ Introduction


۲٫ Results and discussion


۳٫ Materials and methods


Acknowledgements


References

نمونه متن انگلیسی مقاله

Abstract


The habituation of cultured cells to cellulose biosynthesis inhibitors such as dichlobenil (dichlorobenzonitrile, DCB) has proven a valuable tool to elucidate the mechanisms involved in plant cell wall structural plasticity. Our group has demonstrated that maize cells cope with DCB through a modified cell wall in which cellulose is replaced by a more extensive network of highly cross-linked feruloylated arabinoxylans. In order to gain further insight into the contribution of phenolics to the early remodelling of cellulose-deficient cell walls, a comparative HPLC-PAD analysis was carried out of hydroxycinnamates esterified into nascent and cell wall polysaccharides obtained from non-habituated (NH) and habituated to low DCB concentrations (1.5 μM; H) maize suspensioncultured cells. Incipient DCB-habituated cell walls showed significantly higher levels of esterified ferulic acid and p-coumaric acid throughout the culture cycle. In terms of cell wall fortification, ferulic acid is associated to arabinoxylan crosslinking whereas the increase of p-coumaric suggests an early lignification response. As expected, the level of hydroxycinnamates esterified into nascent polysaccharides was also higher in DCB-habituated cells indicating an overexpression of phenylpropanoid pathway. Due to their key role in cell wall strengthening, special attention was paid into the dimerization pattern of ferulic acid. A quantitative comparison of diferulate dehydrodimers (DFAs) between cell lines and cell compartments revealed that an extra dimerization took place in H cells when both nascent and mature cell wall polysaccharides were analysed. In addition, qualitative differences in the ferulic acid coupling pattern were detected in H cells, allowing us to suggest that 8-O-4′-DFA and 8-5′-DFA featured the ferulic acid dimerization when it occurred in the protoplasmic and cell wall fractions respectively. Both qualitative and quantitative differences in the phenolic profile between NH and H cells point to a regioselectivity in the ferulate dehydrodimerization.


Introduction


Primary cell walls of commelinoid monocots (also known as type II cell walls) such as maize are composed of a framework of cellulose microfibrils embedded in a hemicellulosic matrix of (glucurono)arabinoxylans and a smaller amount of pectins and glycoproteins (Carpita and Gibeaut, 1993). These cell walls are also characterised by the presence of cell wall phenolics, mainly the hydroxycinnamates, ferulic (FA) and p-coumaric (CA) acids, which are ester-linked to α–L-arabinosyl residues of (glucurono)arabinoxylans (Smith and Hartley, 1983; Wende and Fry, 1997). Although phenolics are minor components of the primary cell wall (~1% of cell wall dry weight), their contribution to cell wall assembly seems to be crucial. In vitro experiments have demonstrated that hydroxycinnamates are susceptible to oxidative coupling in the presence of peroxidases and hydrogen peroxide, cross-linking adjacent arabinoxylan (AX) molecules and thus contributing to cell wall assembly (Geissman and Neukom, 1971; Fry et al., 2000; Fry, 2004; Encina and Fry, 2005; Parker et al., 2005; Burr and Fry, 2009). Due to its polysaccharide cross-linking role, phenolic coupling is involved in a number of cell wall properties, causing cell wall stiffening and growth cessation, promoting tissue cohesion, strengthening cell wall structure in response to biotic and abiotic stresses and hindering cell wall degradability (de O Buanafina, 2009).

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