مقاله انگلیسی بیولوژی سیستم های متابولیک و چند اشیاء سیانوباکترها: چشم اندازها و جهت های آینده
Abstract
Graphical abstract
Keywords
Introduction
Genome annotation, reconstruction of genome-scale metabolic models (GSMMs), and their applications in cyanobacterial strain designing
Metabolic flux analyses of cyanobacterial metabolism
Metabolomics
Transcriptomics
Proteomics analysis: tools, techniques, and challenges
Integrated omics
Conclusions
CRediT authorship contribution statement
Declaration of Competing Interest
Acknowledgements
References
ABSTRACT
Cyanobacteria are oxygenic photoautotrophs whose metabolism contains key biochemical pathways to fix atmospheric CO2 and synthesize various metabolites. The development of bioengineering tools has enabled the manipulation of cyanobacterial chassis to produce various valuable bioproducts photosynthetically. However, effective utilization of cyanobacteria as photosynthetic cell factories needs a detailed understanding of their metabolism and its interaction with other cellular processes. Implementing systems and synthetic biology tools has generated a wealth of information on various metabolic pathways. However, to design effective engineering strategies for further improvement in growth, photosynthetic efficiency, and enhanced production of target biochemicals, in-depth knowledge of their carbon/nitrogen metabolism, pathway fluxe distribution, genetic regulation and integrative analyses are necessary. In this review, we discuss the recent advances in the development of genome-scale metabolic models (GSMMs), omics analyses (metabolomics, transcriptomics, proteomics, fluxomics), and integrative modeling approaches to showcase the current understanding of cyanobacterial metabolism.