پروفایل سازی ترانسکریپتوم RNA های غیر کد کننده مربوط به بافت چربی
Abstract
1- Introduction
2- Materials and methods
3- Results
4- Discussion
5- Conclusion
References
Abstract
Qinchuan cattle is one of the five yellow cattle breeds in China with good performance of meat. The proliferation and differentiation level of muscle and fat are closely related to the growth and development of the organism and are the key factors affecting the quality of meat. In order to study the effect of lncRNA on the fat tissues of Qinchuan cattle, six calf and adult bovine adipose tissues were selected for high-throughput sequencing. We obtained 3,716 lncRNA candidates from calves and adult cattle fat samples, among them 789 lncRNA were annotated and 2,927 lncRNA were novel lncRNA. A number of lncRNAs were highly abundant, and 119 lncRNA were differentially expressed between two developmental stages. We further validated several differentially expressed lncRNAs using qPCR, and the results were consistent with the sequencing data. Therefore, we conclude that lncRNA may play an important role in adipose tissue in different age groups of cattle.
Introduction
Fat is synthesized from glycerin and fatty acids and is an important part of the organism. As the core of the nutritional value of meat, it has a positive effect on the appearance, texture, flavor, hardness and shelf life of the meat1 . However, the excessive deposition of fat not only affects meat quality, but also risks to human health. Therefore, clarifying the regulation mechanism of lipogenesis is of great significance for improving meat quality. Lipogenesis is a complex and elaborate procedural regulation process involving a series of transcription factors2 . At present, more and more researchers have reported on the biological processes of fat synthesis. 3, 4 Numerous studies have shown that the peroxisome proliferator-activated receptor (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) play a key role in this process. However, lipogenesis involves multiple gene expression, signal transduction, and network regulation. The process is complex and new regulatory factors are constantly being identified. Recent studies have shown that long noncoding RNAs (lncRNAs) also have important regulatory effects on adipogenesis, but there are few studies in cattle.